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Purified blood eosinophils. ( a ) Dot plot of all blood eosinophils labeled with <t>CD62L-FITC</t> antibody, excluding cell debris (with forward scatter/side scatter), and gated using a fixed threshold. ( b ) Fluorescence histogram of all blood eosinophils labeled with <t>CD62L-FITC</t> antibody. ( c ) Merged fluorescence histograms of all blood eosinophils without CD62L-FITC antibodies; iEOS-like and rEOS-like cells labeled with CD62L-FITC antibodies.
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Purified blood eosinophils. ( a ) Dot plot of all blood eosinophils labeled with <t>CD62L-FITC</t> antibody, excluding cell debris (with forward scatter/side scatter), and gated using a fixed threshold. ( b ) Fluorescence histogram of all blood eosinophils labeled with <t>CD62L-FITC</t> antibody. ( c ) Merged fluorescence histograms of all blood eosinophils without CD62L-FITC antibodies; iEOS-like and rEOS-like cells labeled with CD62L-FITC antibodies.
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Purified blood eosinophils. ( a ) Dot plot of all blood eosinophils labeled with CD62L-FITC antibody, excluding cell debris (with forward scatter/side scatter), and gated using a fixed threshold. ( b ) Fluorescence histogram of all blood eosinophils labeled with CD62L-FITC antibody. ( c ) Merged fluorescence histograms of all blood eosinophils without CD62L-FITC antibodies; iEOS-like and rEOS-like cells labeled with CD62L-FITC antibodies.

Journal: Journal of Inflammation Research

Article Title: Effects of Eosinophilopoietins and C-C Chemokines on Chemotaxis, Adhesion, and ROS Production of Blood Eosinophil Subtypes in Asthma Patients

doi: 10.2147/JIR.S564553

Figure Lengend Snippet: Purified blood eosinophils. ( a ) Dot plot of all blood eosinophils labeled with CD62L-FITC antibody, excluding cell debris (with forward scatter/side scatter), and gated using a fixed threshold. ( b ) Fluorescence histogram of all blood eosinophils labeled with CD62L-FITC antibody. ( c ) Merged fluorescence histograms of all blood eosinophils without CD62L-FITC antibodies; iEOS-like and rEOS-like cells labeled with CD62L-FITC antibodies.

Article Snippet: This process utilized magnetic beads conjugated with antibodies against CD62L (L-selectin) (Miltenyi Biotec, Bergisch Gladbach, Germany), a marker expressed mainly by rEOS-like cells, though not on iEOS-like cells., After separation, both cell populations were confirmed using a flow cytometer, FACS Calibur (BD Biosciences, San Jose, CA, USA).

Techniques: Purification, Labeling, Fluorescence